Impact of copper on protein pattern of coelomic fluid in red earthworm
Lumbricus rubellus
A. Zebarjad 1, M. Arabi 1, A.M. Ahadi 2
1 Biology Depat., 2 Genetics Dept.; Faculty of Basic Sciences, Shahrekord University.
Email: mehranarabi@hotmail.com
Abstract
Introduction
Copper ions are well known stressors in both invertebrates and vertebrates. Generally, negative impact of copper was always seen in animal models. Earthworms are one of the best models in immune-toxicological studies as they are abundant and have a unique immune system. In the body extract of them, there is a glycolipoprotein compound viz. G-90 complex (20-70 KDa fractions) that possesses antioxidant, anti-bacterial and mitotic characteristics. It has been shown that G-90 may applied as a medicine to cure some disease particularly in wound healing. In present investigation, red earthworms (Lumbricus rubellus; Lumbricidae) were used as animal model which treated with copper ions as a physico-chemical stressor.
Materials and Methods
In our study, copper sulfate (LD50 = 150 mg/Kg soil) in different concentrations 25, 50 and 100 mg/Kg artificial soil were used for 14 days under constant laboratory conditions. SDS-PAGE electrophoresis technique applied to show possible changes in the coelomic protein pattern for fractions of G-90 complex and also FRAP (ferric reducing antioxidant power) values were used to measure antioxidant power in red earthworm Lumbricus rubellus coelomic fluid.
Results
Our electrophoretic results (using Image J software) showed that in treated groups with copper, the intensity of 20-70 KDa gel bands was increased compared to controls indicating elevation in gene expression of G-90 fractions. FRAP values showed that copper treatment caused an increase in antioxidant power of coelomic fluid to encounter unwanted and abnormal situations e.g. copper stress.
Conclusion
Collectively, copper sulfate stress can potentiate the antioxidant power and also increase the amount of different fractions of G-90 complex in earthworm body under laboratory conditions. Therefore, copper stress can be used as an agent to increase the power of G-90 complex in cure of different diseases.
Keyword: Copper, Red earthworm, Coelomic fluid, G-90, Protein pattern, FRAP.
برچسبها: مقاله ارائه شده, کنگره سم شناسی ایران, علوم پزشکی, داروسازی
